m hominis strain pg21 Search Results


strain m hominis  (ATCC)
95
ATCC strain m hominis
<t>Mycoplasma</t> <t>hominis</t> infection alters the growth and colony formation of primary human keratinocytes (PHKs) even at low inoculum concentrations. ( A ) Quantification of viable PHKs after 72 h of infection with M. hominis at different concentrations. Approximately 8000 PHKs/well were present at the time of infection. * Represents a statistically significant difference between the infection and control groups (one-tailed Mann–Whitney test, significance level of 95%, (*) p ≥ 0.05, (**) p ≥ 0.01, and (***) p ≥ 0.001). ( B ) PHK growth curves after M. hominis infection. Plating was performed with approximately 2.3 × 10 3 to 3.7 × 10 3 cells. After 12–24 h, M. hominis infection was induced at a multiplicity of infection (MOI) of 0.04–0.6. After 24, 48, 72, 96, 120,144, and 168 h of infection, the cells were trypsinised and counted using a haemocytometer. The assays were performed in triplicate. The points on the curves represent the average number of cells obtained with the respective standard deviations. From 48 h of M. hominis infection, a statistically significant difference was observed in the infection group compared with that in the control group (two-way ANOVA with Bonferroni post-test, significance level of 95%). ( C ) The clonogenic assay performed with PHKs after M. hominis infection. The number of PHKs initially plated was approximately 1000. The MOI for M. hominis was 4.44. Left: photograph of the petri dishes in one of the tests. The colonies were stained with crystal violet and counted using the Promega Colony Counter (Promega). Right: reduction in the colony formation percentage after 15 days of infection. One-tailed Mann–Whitney test, significance level of 95%.
Strain M Hominis, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reference strain pg21  (ATCC)
95
ATCC reference strain pg21
<t>Mycoplasma</t> <t>hominis</t> infection alters the growth and colony formation of primary human keratinocytes (PHKs) even at low inoculum concentrations. ( A ) Quantification of viable PHKs after 72 h of infection with M. hominis at different concentrations. Approximately 8000 PHKs/well were present at the time of infection. * Represents a statistically significant difference between the infection and control groups (one-tailed Mann–Whitney test, significance level of 95%, (*) p ≥ 0.05, (**) p ≥ 0.01, and (***) p ≥ 0.001). ( B ) PHK growth curves after M. hominis infection. Plating was performed with approximately 2.3 × 10 3 to 3.7 × 10 3 cells. After 12–24 h, M. hominis infection was induced at a multiplicity of infection (MOI) of 0.04–0.6. After 24, 48, 72, 96, 120,144, and 168 h of infection, the cells were trypsinised and counted using a haemocytometer. The assays were performed in triplicate. The points on the curves represent the average number of cells obtained with the respective standard deviations. From 48 h of M. hominis infection, a statistically significant difference was observed in the infection group compared with that in the control group (two-way ANOVA with Bonferroni post-test, significance level of 95%). ( C ) The clonogenic assay performed with PHKs after M. hominis infection. The number of PHKs initially plated was approximately 1000. The MOI for M. hominis was 4.44. Left: photograph of the petri dishes in one of the tests. The colonies were stained with crystal violet and counted using the Promega Colony Counter (Promega). Right: reduction in the colony formation percentage after 15 days of infection. One-tailed Mann–Whitney test, significance level of 95%.
Reference Strain Pg21, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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d1j7r8 d1j7r8 mychp bacteria alas mho 1310 mycoplasma hominis  (ATCC)
99
ATCC d1j7r8 d1j7r8 mychp bacteria alas mho 1310 mycoplasma hominis
<t>Mycoplasma</t> <t>hominis</t> infection alters the growth and colony formation of primary human keratinocytes (PHKs) even at low inoculum concentrations. ( A ) Quantification of viable PHKs after 72 h of infection with M. hominis at different concentrations. Approximately 8000 PHKs/well were present at the time of infection. * Represents a statistically significant difference between the infection and control groups (one-tailed Mann–Whitney test, significance level of 95%, (*) p ≥ 0.05, (**) p ≥ 0.01, and (***) p ≥ 0.001). ( B ) PHK growth curves after M. hominis infection. Plating was performed with approximately 2.3 × 10 3 to 3.7 × 10 3 cells. After 12–24 h, M. hominis infection was induced at a multiplicity of infection (MOI) of 0.04–0.6. After 24, 48, 72, 96, 120,144, and 168 h of infection, the cells were trypsinised and counted using a haemocytometer. The assays were performed in triplicate. The points on the curves represent the average number of cells obtained with the respective standard deviations. From 48 h of M. hominis infection, a statistically significant difference was observed in the infection group compared with that in the control group (two-way ANOVA with Bonferroni post-test, significance level of 95%). ( C ) The clonogenic assay performed with PHKs after M. hominis infection. The number of PHKs initially plated was approximately 1000. The MOI for M. hominis was 4.44. Left: photograph of the petri dishes in one of the tests. The colonies were stained with crystal violet and counted using the Promega Colony Counter (Promega). Right: reduction in the colony formation percentage after 15 days of infection. One-tailed Mann–Whitney test, significance level of 95%.
D1j7r8 D1j7r8 Mychp Bacteria Alas Mho 1310 Mycoplasma Hominis, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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quantitative mycoplasma genomic dna  (ATCC)
93
ATCC quantitative mycoplasma genomic dna
<t>Mycoplasma</t> <t>hominis</t> infection alters the growth and colony formation of primary human keratinocytes (PHKs) even at low inoculum concentrations. ( A ) Quantification of viable PHKs after 72 h of infection with M. hominis at different concentrations. Approximately 8000 PHKs/well were present at the time of infection. * Represents a statistically significant difference between the infection and control groups (one-tailed Mann–Whitney test, significance level of 95%, (*) p ≥ 0.05, (**) p ≥ 0.01, and (***) p ≥ 0.001). ( B ) PHK growth curves after M. hominis infection. Plating was performed with approximately 2.3 × 10 3 to 3.7 × 10 3 cells. After 12–24 h, M. hominis infection was induced at a multiplicity of infection (MOI) of 0.04–0.6. After 24, 48, 72, 96, 120,144, and 168 h of infection, the cells were trypsinised and counted using a haemocytometer. The assays were performed in triplicate. The points on the curves represent the average number of cells obtained with the respective standard deviations. From 48 h of M. hominis infection, a statistically significant difference was observed in the infection group compared with that in the control group (two-way ANOVA with Bonferroni post-test, significance level of 95%). ( C ) The clonogenic assay performed with PHKs after M. hominis infection. The number of PHKs initially plated was approximately 1000. The MOI for M. hominis was 4.44. Left: photograph of the petri dishes in one of the tests. The colonies were stained with crystal violet and counted using the Promega Colony Counter (Promega). Right: reduction in the colony formation percentage after 15 days of infection. One-tailed Mann–Whitney test, significance level of 95%.
Quantitative Mycoplasma Genomic Dna, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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m hominis atcc 23114dtm dna  (ATCC)
91
ATCC m hominis atcc 23114dtm dna
<t>Mycoplasma</t> <t>hominis</t> infection alters the growth and colony formation of primary human keratinocytes (PHKs) even at low inoculum concentrations. ( A ) Quantification of viable PHKs after 72 h of infection with M. hominis at different concentrations. Approximately 8000 PHKs/well were present at the time of infection. * Represents a statistically significant difference between the infection and control groups (one-tailed Mann–Whitney test, significance level of 95%, (*) p ≥ 0.05, (**) p ≥ 0.01, and (***) p ≥ 0.001). ( B ) PHK growth curves after M. hominis infection. Plating was performed with approximately 2.3 × 10 3 to 3.7 × 10 3 cells. After 12–24 h, M. hominis infection was induced at a multiplicity of infection (MOI) of 0.04–0.6. After 24, 48, 72, 96, 120,144, and 168 h of infection, the cells were trypsinised and counted using a haemocytometer. The assays were performed in triplicate. The points on the curves represent the average number of cells obtained with the respective standard deviations. From 48 h of M. hominis infection, a statistically significant difference was observed in the infection group compared with that in the control group (two-way ANOVA with Bonferroni post-test, significance level of 95%). ( C ) The clonogenic assay performed with PHKs after M. hominis infection. The number of PHKs initially plated was approximately 1000. The MOI for M. hominis was 4.44. Left: photograph of the petri dishes in one of the tests. The colonies were stained with crystal violet and counted using the Promega Colony Counter (Promega). Right: reduction in the colony formation percentage after 15 days of infection. One-tailed Mann–Whitney test, significance level of 95%.
M Hominis Atcc 23114dtm Dna, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mycoplasma hominis  (ATCC)
90
ATCC mycoplasma hominis
<t>Mycoplasma</t> <t>hominis</t> infection alters the growth and colony formation of primary human keratinocytes (PHKs) even at low inoculum concentrations. ( A ) Quantification of viable PHKs after 72 h of infection with M. hominis at different concentrations. Approximately 8000 PHKs/well were present at the time of infection. * Represents a statistically significant difference between the infection and control groups (one-tailed Mann–Whitney test, significance level of 95%, (*) p ≥ 0.05, (**) p ≥ 0.01, and (***) p ≥ 0.001). ( B ) PHK growth curves after M. hominis infection. Plating was performed with approximately 2.3 × 10 3 to 3.7 × 10 3 cells. After 12–24 h, M. hominis infection was induced at a multiplicity of infection (MOI) of 0.04–0.6. After 24, 48, 72, 96, 120,144, and 168 h of infection, the cells were trypsinised and counted using a haemocytometer. The assays were performed in triplicate. The points on the curves represent the average number of cells obtained with the respective standard deviations. From 48 h of M. hominis infection, a statistically significant difference was observed in the infection group compared with that in the control group (two-way ANOVA with Bonferroni post-test, significance level of 95%). ( C ) The clonogenic assay performed with PHKs after M. hominis infection. The number of PHKs initially plated was approximately 1000. The MOI for M. hominis was 4.44. Left: photograph of the petri dishes in one of the tests. The colonies were stained with crystal violet and counted using the Promega Colony Counter (Promega). Right: reduction in the colony formation percentage after 15 days of infection. One-tailed Mann–Whitney test, significance level of 95%.
Mycoplasma Hominis, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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m hominis  (ATCC)
93
ATCC m hominis
<t>Mycoplasma</t> <t>hominis</t> infection alters the growth and colony formation of primary human keratinocytes (PHKs) even at low inoculum concentrations. ( A ) Quantification of viable PHKs after 72 h of infection with M. hominis at different concentrations. Approximately 8000 PHKs/well were present at the time of infection. * Represents a statistically significant difference between the infection and control groups (one-tailed Mann–Whitney test, significance level of 95%, (*) p ≥ 0.05, (**) p ≥ 0.01, and (***) p ≥ 0.001). ( B ) PHK growth curves after M. hominis infection. Plating was performed with approximately 2.3 × 10 3 to 3.7 × 10 3 cells. After 12–24 h, M. hominis infection was induced at a multiplicity of infection (MOI) of 0.04–0.6. After 24, 48, 72, 96, 120,144, and 168 h of infection, the cells were trypsinised and counted using a haemocytometer. The assays were performed in triplicate. The points on the curves represent the average number of cells obtained with the respective standard deviations. From 48 h of M. hominis infection, a statistically significant difference was observed in the infection group compared with that in the control group (two-way ANOVA with Bonferroni post-test, significance level of 95%). ( C ) The clonogenic assay performed with PHKs after M. hominis infection. The number of PHKs initially plated was approximately 1000. The MOI for M. hominis was 4.44. Left: photograph of the petri dishes in one of the tests. The colonies were stained with crystal violet and counted using the Promega Colony Counter (Promega). Right: reduction in the colony formation percentage after 15 days of infection. One-tailed Mann–Whitney test, significance level of 95%.
M Hominis, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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23114dq  (ATCC)
90
ATCC 23114dq
<t>Mycoplasma</t> <t>hominis</t> infection alters the growth and colony formation of primary human keratinocytes (PHKs) even at low inoculum concentrations. ( A ) Quantification of viable PHKs after 72 h of infection with M. hominis at different concentrations. Approximately 8000 PHKs/well were present at the time of infection. * Represents a statistically significant difference between the infection and control groups (one-tailed Mann–Whitney test, significance level of 95%, (*) p ≥ 0.05, (**) p ≥ 0.01, and (***) p ≥ 0.001). ( B ) PHK growth curves after M. hominis infection. Plating was performed with approximately 2.3 × 10 3 to 3.7 × 10 3 cells. After 12–24 h, M. hominis infection was induced at a multiplicity of infection (MOI) of 0.04–0.6. After 24, 48, 72, 96, 120,144, and 168 h of infection, the cells were trypsinised and counted using a haemocytometer. The assays were performed in triplicate. The points on the curves represent the average number of cells obtained with the respective standard deviations. From 48 h of M. hominis infection, a statistically significant difference was observed in the infection group compared with that in the control group (two-way ANOVA with Bonferroni post-test, significance level of 95%). ( C ) The clonogenic assay performed with PHKs after M. hominis infection. The number of PHKs initially plated was approximately 1000. The MOI for M. hominis was 4.44. Left: photograph of the petri dishes in one of the tests. The colonies were stained with crystal violet and counted using the Promega Colony Counter (Promega). Right: reduction in the colony formation percentage after 15 days of infection. One-tailed Mann–Whitney test, significance level of 95%.
23114dq, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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paesel lorei pg21  (Paesel Lorei GmbH Co KG)
90
Paesel Lorei GmbH Co KG paesel lorei pg21
Prevalence of AR low/negative Expression Pattern in CaP and CRPC.
Paesel Lorei Pg21, supplied by Paesel Lorei GmbH Co KG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pg-21 photoplethysmography (ppg) probe  (Hadeco Inc)
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Hadeco Inc pg-21 photoplethysmography (ppg) probe
Prevalence of AR low/negative Expression Pattern in CaP and CRPC.
Pg 21 Photoplethysmography (Ppg) Probe, supplied by Hadeco Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti ar pg-21  (Merck KGaA)
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Merck KGaA rabbit anti ar pg-21
Prevalence of AR low/negative Expression Pattern in CaP and CRPC.
Rabbit Anti Ar Pg 21, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Mycoplasma hominis infection alters the growth and colony formation of primary human keratinocytes (PHKs) even at low inoculum concentrations. ( A ) Quantification of viable PHKs after 72 h of infection with M. hominis at different concentrations. Approximately 8000 PHKs/well were present at the time of infection. * Represents a statistically significant difference between the infection and control groups (one-tailed Mann–Whitney test, significance level of 95%, (*) p ≥ 0.05, (**) p ≥ 0.01, and (***) p ≥ 0.001). ( B ) PHK growth curves after M. hominis infection. Plating was performed with approximately 2.3 × 10 3 to 3.7 × 10 3 cells. After 12–24 h, M. hominis infection was induced at a multiplicity of infection (MOI) of 0.04–0.6. After 24, 48, 72, 96, 120,144, and 168 h of infection, the cells were trypsinised and counted using a haemocytometer. The assays were performed in triplicate. The points on the curves represent the average number of cells obtained with the respective standard deviations. From 48 h of M. hominis infection, a statistically significant difference was observed in the infection group compared with that in the control group (two-way ANOVA with Bonferroni post-test, significance level of 95%). ( C ) The clonogenic assay performed with PHKs after M. hominis infection. The number of PHKs initially plated was approximately 1000. The MOI for M. hominis was 4.44. Left: photograph of the petri dishes in one of the tests. The colonies were stained with crystal violet and counted using the Promega Colony Counter (Promega). Right: reduction in the colony formation percentage after 15 days of infection. One-tailed Mann–Whitney test, significance level of 95%.

Journal: Microorganisms

Article Title: Mycoplasma hominis Causes DNA Damage and Cell Death in Primary Human Keratinocytes

doi: 10.3390/microorganisms10101962

Figure Lengend Snippet: Mycoplasma hominis infection alters the growth and colony formation of primary human keratinocytes (PHKs) even at low inoculum concentrations. ( A ) Quantification of viable PHKs after 72 h of infection with M. hominis at different concentrations. Approximately 8000 PHKs/well were present at the time of infection. * Represents a statistically significant difference between the infection and control groups (one-tailed Mann–Whitney test, significance level of 95%, (*) p ≥ 0.05, (**) p ≥ 0.01, and (***) p ≥ 0.001). ( B ) PHK growth curves after M. hominis infection. Plating was performed with approximately 2.3 × 10 3 to 3.7 × 10 3 cells. After 12–24 h, M. hominis infection was induced at a multiplicity of infection (MOI) of 0.04–0.6. After 24, 48, 72, 96, 120,144, and 168 h of infection, the cells were trypsinised and counted using a haemocytometer. The assays were performed in triplicate. The points on the curves represent the average number of cells obtained with the respective standard deviations. From 48 h of M. hominis infection, a statistically significant difference was observed in the infection group compared with that in the control group (two-way ANOVA with Bonferroni post-test, significance level of 95%). ( C ) The clonogenic assay performed with PHKs after M. hominis infection. The number of PHKs initially plated was approximately 1000. The MOI for M. hominis was 4.44. Left: photograph of the petri dishes in one of the tests. The colonies were stained with crystal violet and counted using the Promega Colony Counter (Promega). Right: reduction in the colony formation percentage after 15 days of infection. One-tailed Mann–Whitney test, significance level of 95%.

Article Snippet: The strain M. hominis (ATCC 23114-PG-21) was obtained from the ICB/USP Mycoplasma laboratory and grown in 100 mL of SP4 medium [ ] until the mid-log phase.

Techniques: Infection, Control, One-tailed Test, MANN-WHITNEY, Clonogenic Assay, Staining

Prevalence of AR low/negative Expression Pattern in CaP and CRPC.

Journal: Neoplasia (New York, N.Y.)

Article Title: Development and prevalence of castration-resistant prostate cancer subtypes

doi: 10.1016/j.neo.2020.09.002

Figure Lengend Snippet: Prevalence of AR low/negative Expression Pattern in CaP and CRPC.

Article Snippet: Hobisch et al. , 1996 , Mets , 33.3% , 12 , Paesel Lorei PG21.

Techniques: Expressing